Kalinova M. Microgametophytic selection for cold tolerance in spring rape.

Reseach subjects: spring rapeseed. Reseach objectives: to study the influence of selection in F1 of microgametophytes, tolerant to low temperature, on the structure and quality of the resulted sporophytic generation in spring rapeseed. Methods: microgametophytic selection, genetic analysis. Theoretical and practical results: the relationship between cold tolerance of sporophyte and gametophyte was shown. The method of selection of cold tolerant genotypes at the level of pollen was developed. The method of the evaluation of cold tolerance of a sporophyte by its male gametophyte was offered. Novelty of introduced: the close relationship between cold tolerance of sporophyte and gametophyte in spring rapeseed was revealed. For the first time on this crop the data on influence of temperature treatment of microgametophytes (at different stages of their development) in F1 on the structure and quality of segregating F2 populations were obtained. It was established that treatment of microgametophytes with low temperature both at the stage of mature pollen and during pollen germination and pollen tube growth in F1 hybrids increased the cold tolerance of the resulted sporophytic generation. Implementation degree: the cold tolerant forms of spring rape-seed were developed. Effectiveness: acceleration of breeding for cold tolerance at the account of a faster selection of cold tolerant genotypes at the level of pollen. Sphere, sector of implementation: spring rape breeding.