Kajda I. Development and characterisation of new replicating vector and packaging cells for retroviral gene transfer

Українська версія

Thesis for the degree of Candidate of Sciences (CSc)

State registration number

0400U000318

Applicant for

Specialization

  • 03.00.03 - Молекулярна біологія

18-01-2000

Specialized Academic Board

Д 26.237.01

Institute of Molecular Biology and Genetics of NAS of Ukraine

Essay

New replicating vector tddaPr-RSV-Ce- and new helper virus for packaging cells F18/pJD were created on the base of transformation-defective mutant duck-adapted variant of avian Rous sarcoma virus Prague strain subgroup C (td daPr-RSV-C) with the extended host range. The use of adapted variant avian RSV as the base for helper virus allowed to obtain a high productivity packaging cells F18/pJD (titer 106 CFU/ml) and to avoid of the replicate competent virus appearance in a same time.

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