Petrosyan A. Biotesting of marine water and botton seadiments of the Norh-Western part of the Black Sea

1. Natural seawater and bottom sediments in the northwest part of the Black sea.Elaboration and approbation of methods of bioassays of water, bottom sediments and separate pollutants for conditions of a northwest part of the Black sea. Methods of bioassays of a marine environment with usage of laboratory cultures Phaeodactylum tricornutum Bochlin., Euplotes patella lemani Dragesco and nauplii of Artemia salina L.There are established seasonal and spatial oscillations of toxicity level of natural seawater and bottom sediments in the Odessa Bay and adjoining water areas. The scale of definition of toxicity of tested mediums is offered. For the first time by results of bioassays "hot spots" are detected, on which toxicity was fixed for a number of years and in different seasons of a year. For the first time with the help of methods of bioassays there was carried out an analysis of toxicity of natural seawater, sewages of a different origin threw into the northwest part of the Black Sea, separate contamin ants, bottom sediments in the contaminated marine water areas and loose goods. A.G.Petrosyan worked out methods of bioassays for seawaters with a varied salinity and water extracts of bottom sediments with usage of laboratory cultures Phaeodactylum tricornutum Bohlin., Euplotes patella lemani Dragesco and nauplii Artemia salina L. The methods are included into the Managing normative document 211.1.4.047-95 "Bioassays of marine water and sewages that are exported into the sea", approved by Nature Department of Ukraine № 46 (May, 30, 1995). The offered complex of methods for toxicity assessment of water, sewages of a different origin, separate contaminants and bottom sediments (that present potential danger to the sea) is used by National revision of preservation of the Black sea in its observations of quality of a marine environment. Hydrobiology, ecology, water toxicology