Budkevych T. Specific features of the interactions between elongation factor 1alpha and others components of the eukaryotic translational apparatus

Українська версія

Thesis for the degree of Candidate of Sciences (CSc)

State registration number

0400U003112

Thesis Registration Form

0400U003112.pdf

Applicant for

Specialization

  • 03.00.03 - Молекулярна біологія

21-11-2000

Specialized Academic Board

Д 26.237.01

Institute of Molecular Biology and Genetics of NAS of Ukraine

Essay

The direct experimental evidences of the in vitro existence of non-canonical complexes EF-1alpha in GDP- and GTP-bound forms with deacylated tRNA and phenylalanyl-tRNA synthetase are presented. The Kd of [EF-1alpha*GDP*tRNA] and [EF-1alpha*GTP*tRNA] was determined. The regions of rabbit liver tRNAPhe, tRNALeu and tRNATyr involved in the interaction with EF-1alpha*GDP/GTP have been revealed. For the first time the solution conformation of rabbit liver EF-1alpha*GDP and its complex with tRNAVal has been studied by small-angle neutron scattering SANS). The model of the EF-1alpha-mediated tRNA/aa-tRNA channeling during eukaryotic protein biosynthesis was proposed.

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