Borysov S. Ca2+ and chromatin fragmentation in rat thymus and spleen lymphocyte nuclei during radiation-induced apoptosis

Українська версія

Thesis for the degree of Candidate of Sciences (CSc)

State registration number

0400U003370

Thesis Registration Form

0400U003370.pdf

Applicant for

Specialization

  • 03.00.04 - Біохімія

18-12-2000

Specialized Academic Board

Д 26.001.24

Taras Shevchenko National University of Kyiv

Essay

The dissertation is devoted to investigation of chromatin fragmentation, cytosolic and intranuclear Ca2+ concentration and nuclear Ca2+-transport in thymus and spleen lymphocytes during radiation-induced apoptosis. Using methods of DNA electrophoresis in agarose gel, investigation of Ca2+ concentration using Ca2+ fluorescent dyes fura-2 and indo-1, investigation of Ca2+-transport using 45Ca2+ and others, it has been shown that in lymphocyte nucleus exists an ATP-dependent Ca2+-transporting system. The results suggest that mechanisms of radiation-induced apoptosis in thymocytes and spleenocytes are different, besides it, the results extend our idea of nuclear Ca2+-regulation. Presented data can be used in development of methods to control lympocyte apoptotic death.

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