Sokol O. Tryptophan-2,3-dioxygenase activity and content of cytochrome Р-450 in the rat liver after mercury chloride and cobalt chloride administration.

The object of study - blood and liver of rats. The aim of study is devoted to study a tryptophan-2,3-dioxygenase activity and content of cytochrome Р-450 in the rat liver after mercury chloride and cobalt chloride administration; research of prevention possibility or limitation of toxic action HgCl2 and CoCl2 by preliminary administration of a-tocopherol, bilirubin or L-tryptophan. Methods - biochemical: determination of enzyme activity, differential spectrophotometry, differential centrifugation, and determination of protein content. Is shown that there is a direct relation of a content of cytochrome Р-450 content from ability of tryptophan-2,3-dioxygenase to bind excess of a heme in the cytosole of hepatocytes. Comparison of influence HgCl2 and CoCl2 on tryptophan-2,3-dioxygenase activity and the content of cytochrome P-450 has revealed key differences in response from the side tryptophan-2,3-dioxygenase and cytochrome P-450, that points different mechanisms of an action HgCl2 and CoCl2 on these hemo proteins. Field of application - scientific institutions of a medicobiological profile, because the results of research can be utilised in basic researches for development of models and approaches of prophylaxis of poisons of salts of heavy metals; in educational process on pharmacology and biological chemistry.