Yasinska I. Purification, catalytic properties and activity regulation mechanisms of cytochrome P450 aromatase

Українська версія

Thesis for the degree of Candidate of Sciences (CSc)

State registration number

0402U000809

Thesis Registration Form

0402U000809.pdf

Applicant for

Specialization

  • 03.00.04 - Біохімія

18-03-2002

Specialized Academic Board

Д 26.001.24

Taras Shevchenko National University of Kyiv

Essay

Research object: сytochrome P450 aromatase, rats, healthy people. To study the in vitro effects of reductors-antioxidants, cortisol, polychlorinated biphenyls, genistein and quercetin on the aromatase activity and in vivo effects of some of them on the enzyme activity and estrogen formation. Methods: affinity precipitation, spectrophotometry, electrophoresis, centrifugation. Established that antioxidants are the aromatase activators and polychlorinated byphenyls as well as genistein and quercetin are its inhibitors in the in vitro and in vivo studies. Cortisol activates aromatase only in vivo. Activity of aromatase, nitric oxide synthase and S-nitrosothiols quantity were found to be decreased in rat organism under conditions of oxidative stress. These data are significant for studies of estrogen biosynthesis regulation. Field of application - scientific institutions for medical and biological studies.

Read more

Files

ConclusionUkr.doc

IntrodUKr.doc

LiterUkr.doc

MethodsUkr.doc

Newlit.doc

ResultsUkr.doc

SoderzhUkr.doc

Spisoksokr.doc

TitleUkr.doc

VyvodyUKR.doc

aref.doc

Similar theses

0524U000046

Oksana V. Melnyk

Peculiarities of free-radical, immune, Ca2+- and NO-dependent regulatory systems of cells and their relationship with the microbiome under conditions of development of male infertility and concomitant autoimmune pathology

0422U100167

Lylyk Mariia Petrivna

Protective action of alpha ketoglutarate in Drosophila melanogaster for exposure to xenobiotics

0422U100159

Semaniuk Uliana Vasylivna

Regulation of physiological and biochemical traits of Drosophila melanogaster by diet and insulin-like peptides

0422U100023

Kotliar Iryna Petrivna

Biochemical mechanisms of anti-inflammatory action of pyrrole derivatives in ulcerative colitis

0521U102076

Danylovych Hanna Victorivna

Biosynthesis and regulatory role of nitric oxide in smooth muscle mitochondria