Kavok N. Age specifity of thyroid hormones rapid effects on lipid metabolism in rat liver

The object of study: the molecular mechanism of thyroid hormones action. Aim: age specifity elucidation of thyroid hormones effect on glycerolipid metabolism in rat liver. Method of investigation: cell biology, biochemical and radioisotope methods. Results, novelty: it was found that L-thyroxine stimulation of the 3-month old rats liver cells rapidly increase diacylglycerol content. Diacylglycerol formation in the stimulated cell is biphasic. Phosphatidyletanolamine and phosphatidylcholine are the precursors of the L-thyroxine-generated diacylglycerol. Diacylglycerol The results obtained provide the demonstration of phospholipase D implication in L-thyroxine induced diacylglycerol formation. In liver cells of 24-month old rats, diacylglycerol and individual phospholipid content were unaffected by hormones. The present data point to an existence of relationship between the deficiency of lipid second messenger generation and age-dependent changes in formation of cellular responses. Field of implementation: fundamental and clinical endocrinology and lipidology.