Romanko M. Lipid peroxidation and its regulation in animal organism in conditions of radionuclide effect and enterosorbtion

Object - biochemical mechanisms of alimentary effect of 137Cs and enterosorbents. Purpose - to study the lipid peroxidation (LPO) intensity, the state of antioxidant (AOS) and monooxygenase (MOS) systems in rat tissues and blood of farming animals in the dynamics of 137Cs and enterosorbents action. Methods - biochemical; radiological with the use of gamma-spectrometrical systems (ORTEC, BIRCON, USА). It was shown that LPO intensification took place in blood and liver of rats after 22 days of 137Cs effect; in blood of cows and bull-calves increase of lipid hydroperoxides content was already recorded at early stages of taking the 137Cs contaminated fodder. LPO activation was followed by the depression of AOS activity in blood of rats, cows and bull-calves and adaptive activation of its enzymatic part and MOS in rat liver. The abilities of different sorbents to prevent from LPO activation, specifically influence on some parameters of AOS and MOS and to restore the lever of lipid exchange indices and to normalize the activity of hepato-specific enzymes in animal tissues to control values were determined. All these facts points out the effective deceleration of development of intoxication of radionuclide etiology. Field of application - the advisability of use of the natural sorbents in live - stock farming at the radionuclides contaminated territories was shown; the results of the study on determination of biochemical mechanisms of alimentary taken 137Cs effect may be the basis for the elaboration of scientifically grounded schemes of detoxication with the application of enterosorbtion.