Kishko T. The role of xanthinoxidase-xanthindehydrogenase system in the mechanism of action of xenobiotics

The object of investigation: rat thymocytes. The aim of work: It the investigation of xanthinoxidase-xanthindehydrogenase (XO-XD) system in the mechanism of toxical action of xenobiotics. Methods: biochemical, biophisical cytological and mathematical statistic methods. It has been, established on rat thymocytes that under action of papaverine and such NO donors as nitrite, nitrate and nitroprusside (NP), N-nitrozodimethylamine (NDMA) the transvertion of xanthindehydrogenase to xanthinoxidase and increase of lipid peroxidation took place. It was shown that NO partially (NaNO2-, НДМА) or completely (NP) determined the toxical action of xenobiotics - NO donors. The toxical influence of NaNO3- is connected with its reduction to NaNO2- , due to action of XO-XD system. Aria of application - scientific medical and biological institution biochemical.