Samokhin A. Total neutral proteinases activity, calpains and a-2-macroglobulin activity at oxidative stress in rats

Objects of investigation - Proteolyse at oxidative stress. Aim of investigation - Peculiarity determination of some components of proteinase and their inhibitors system reaction at oxidative stress, which caused by introduction of cobalt or mercury chloride. Methods - Spectrophotometrical determination of enzymes and their inhibitors activity. Results, novelty - It was shown that the introduction of cobalt chloride to rats causes significant changes in total neutral proteinases and a-2-macroglobulin activity in serum, lungs, heart, liver and kidneys of rats. It has been established that in these conditions the neutral proteinase activity increase is accompanied by significant decrease in a-2-macroglobulin activity. At introduction of mercury chloride it was shown, that the activation of neutral proteinases in serum, liver and kidneys does not lead to a-2-macroglobulin activity decreasing, on the contrary, the increase of its activity is observed. Calpain activity was increased in all reseached organs under cobalt chloride introduction and only in kidney under mercury chloride introduction. It has been revealed that the preliminary introduction of antiinflammatory preparations - pentoxifylline and quercetin prevents the neutral proteinase and a-2-macroglobulin activity changes. It was shown that protein synthesis inhibitor - cycloheximide in concentration, which causes the development of oxidative stress, leads to the activation of researched proteinases and a-2-macroglobulin activity decrease; the preliminary pentoxifylline introduction prevents these changes. Field of application - biochemistry.