Stepnevskaya Y. The physical-chemistry of methods separation and deter-mination of sum of alkaloids of Chelidonium Majus L.

Object of investigation: the separation and determination of property of associates of alkaloids of Chelidonium Majus L, with 12- molybdophosphoric as analytical forms. Aim of investigation: the separation and determination of sum and individual of alkaloids of Chelidonium Majus L for physical-chemistry methods with molybdenphosphat-anion. Methods of investigation: the spectroscopy, amperometry, electrophoreses, the direct potentiometry, chromatography and elec-trolyses. Theoretical and practical results: the dissertation is devoted to elaborating of separation and determination methods of sum of Chelidonium Majus L alkaloids and alkaloids berberin, helidonin. The influence of number of the factors on the separation of sum of alkaloids of Chelidonium Majus L. was studied and the mechanism of formation of sum alkaloids on the electrode was proposed. It was studied the influence of number of the factors on the electrochemical and analytical characteristics of ionselective electrodes (ISE), convertible tocations of alkaloids of Chelidonium Majus L., which account allows to predict the properties of membrane ISE and to develop electrodes with the given characteristics.The complex of sensitive, express techniques of alkaloids of Chelidonium Majus L. definition in the medicinal forms and solutions by the direct potentiometry, electrophoresis, chro-matography and amperometric titration is offered. Degree of inculcation: the developed methods have been introduced into the practice of work of State scientific-research laboratory of control quality of medicinal preparation. Field of application: pharmaceutical and analytical chemistry of bioactive substances.