Maksymjuk H. "Role of calcium, potassium, sodium ions and transport ATPase in preservation of biological usefulness of spermatozoa"

The thesis is devoted to the investigation of influence of Ca2+, Na+, K+ different concentration level in plasma and spermatozoa in human sperm ejaculates and influence Na+,K+-, Ca2+,Mg 2+-ATPase activity on the biological usefulness of reproduction cells. Under normo- and oligospermia. We investigated the peculiarity of content, distribution, ratio and transport of ions and ATPase activity in the "cell-medium" system under exogenous and endogenous effect. Different total levels (high - 31, average - 21 and low 13mM) of K+ concentration and different ratio between the same and opposite ion pairs cause asymmetric distribution of Ca2+, Na+, K+ between plasma and spermatozoa. Significant change of ions concentration and increase of ATPAse activity under oligozoospermia lead to raise or reduction of spermatozoa concentration, reduction of motility and raise the number of pathological forms. After long-term keeping of sperm under optimal and low temperature the method of ions transport changes, namely, inevery research group of ejaculates the scheme of motion vector change was detected. Basing on the experimental results the subject of relation between ions content, transport ATPase activity and biological usefulness of spermatozoa under normo- and oligozoospermia. Key words: Calcium, potassium, sodium ions, Na+,K+-, Ca2+,Mg2+-ATPase, content, distribution, ratio and transport of ions, ejaculate, plasma, spermatozoa, normo- oligozoospermia.