Kachula S. The methabolism of xenobiotics - the substrates of cytochrome P450 in conditions of starvation and administration of inductors and inhibitors

The objects of the research: liver, lungs and kidneys of rats in conditions of starvation and administration of inductors and inhibitors. The purpose of work: definition of the approaches to rising resistance of an organism to adverse influence xenobiotics in conditions of a starvation. Methods: biochemical, pharmacological. Results: in this work is postulated, that activities of cytochromes Р4502Е1 and 3А, during a starvation in a liver, lungs and kidneys of rats increases, but the activity of Р4502D, 2C, 1A2, UDP-glucuronosyltransferase, phenolsulfotransferase and glutathione-S-transferase are reduced. The starvation enhances Р4502E1-dependent stages of a methabolism of acetanilide, bromobenzene, toluene, but inhibits Р4502С- dependent reactions of a metabolism of toluene and indomethacin. Starvation, as well as the administration of acetone and phenobarbital enhance toxicity of bromobenzene and CCl4, but substrates of conjugation and the inhibitors P4502E1 - decrease them. The drug of garlic - INOD'AIL has protective abilities, which are stipulated by inhibition Р4502Е1-dependent on amplification the toxicity of xenobiotics and their detoxication by means of conjugation. Degree of introduction: the results of research are introduced into work of faculties of biochemistry and pharmacology of Vinnitsa National Medical University by M.I.Pirogov, Ukrainian State Scientific and Research Institution of an aftertreatment of the invalids, Vinnitsa, faculties of biochemistry of the Ternopil State Medical Academy by I.YA. Horbacnevsky. The area of application: medical biochemistry.