Pinchuk A. Peculiarities of micropropagation and adaptation of poplar gray poplar white hybrid's planting material (Populus canescens Sm. х Populus alba L.)

Object - heterogeneous cellular populations (callus), received as result of cells differentiation in culture in vitro out of somatic tissue of leaf or other origin of a hybrid of a poplar, meristem sites. Methods - cultivation of somatic cells of plants in vitro which are based on their ability to function on artificial medium in aseptic conditions, and opportunities of an induction of morphogenesis of callus somatic cells. Regenerated plants - were cultivated in green-room's conditions, as well as glasshouses and an open ground nursery. For calculation of quantity of chromosomes in regenerated plants cytology methods was applied. Novelty - comparative studying of morphogenesis potentials of tissues and organs of poplar gray ? a poplar white hybrid (Populus canescens Sm. ? Populus alba L.) is carried out for the first time in vitro. It is shown reaction of plants to factors (exogenous and endogenous) which regulate morphogenesis in vitro. Ways of in vitro morphogenesis of vegetative buds of a hybrid ofa poplar were investigated. For the first time the cytology analysis of regenerated plants and the establishment of chromosomes quantity was carried out. Results - the received data have scientific and practical value in expansion of knowledge about morphogenic potentials of tissues and organs of a hybrid of a poplar gray х a poplar white (Populus canescens Sm. х Populus alba L.). Results of researches can be used for the organization of mass production of a landing material from regenerated plants for creation forest plantations. Behind results of researches methodical recommendations for microclonal propagation of wood and grassy plants are given. Materials of researches also are used in educational process for teaching of experts at carrying out of laboratory employment for disciplines "Biotechnology of plants " and " Wood cultures ". Branch - forestry.