Shkavolyak A. Identification and biochemical characteristic of Na/Li-countertransport in pancreatic beta-cells

Object - Na transport sуstems; methods - biochemical, phisiological, morphological, statistics; news - demonstrated the possibility of realization of Na/Li-countertransport mechanism in rat pancreatic beta-cells of Langerhans; results - data were analysed standard Na/Li countertransport activity and its maximum velocity, constant affinity (Km) for extracellular Na+. In this experimental study was to investigate the effect of pH, temperature and osmolality in extracellular environment, inrtaccellular and extracellular sodium ion concentration on these kinetic parametrs of ionic exchange. Intensivity of this exchange in rat beta-cells is higher than in rat erythrocytes and healthy human erythrocytes. The long-term effect of illumination with helium neon laser at a low doses have been shown oppression of maximum velocity of Na/Li countertransport in beta-cells; introduction - in educative establishments of medical; branch - biochemical, phisiology, membranology, photobiology.