Korobova O. Functional role of myoglobin and carnosine in a nitric oxide cycle during hypoxia

The investigation object is nitric oxide formation by myoglobin following nitritreductase mechanism, as well as the effect of carnosine on this process and myoglobin synthesis under hypoxia of different origin. The work objective is to find out carnosine participation in the process of nitric oxide metabolites conversion by myoglobin under the hypoxia. Methods: spectrophotometry, colorimetry, molecular hybridization, chromatography. It is shown that under hypoxia of various etiology there occurs the decrease of mRNA myoglobin. The process of nitrosilation of RNAse and its substrates is one of the causes of the above process. Carnosine administration prevents from the decrease of the amount of the myoglobin synthesis precursor, activates nitrite reductase activity and inhibits nitrosilation of desoximyoglobin in vitro. Field of application - medical and biological scientific-research and educational institutions.