Sedova K. Mechanisms of combined tolerance to the influence of high-density metals and high temperature

Українська версія

Thesis for the degree of Candidate of Sciences (CSc)

State registration number

0405U000458

Thesis Registration Form

0405U000458.pdf

Applicant for

Specialization

  • 03.00.04 - Біохімія

26-01-2005

Specialized Academic Board

Д 64.051.17

Essay

Research subject: Dunaliella viridis cell culture. Research objective: modelling combined tolerance to high temperature and copper ions revealed by Dunaliella viridis cells, researching mechanisms of combined tolerance to these factors. Research methods - spectrophotometric, electrophoretic methods; determination of protein synthesis rate using radioactive isotopes, determination of copper content by means of atomic absorptive spectrophotometer. It has been shown that Dunaliella viridis cells adapted to high concentration of copper ions also reveal steady tolerance to high temperature. Adaptation to these factors is based upon protein and nucleic acids metabolism changes as well as upon membrane strength changes. A hypothesis has been raised relating to the cooperative nature of changes in metabolic systems of cells in the process of combined tolerance formation.

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