Davydova N. The biochemical mechanisms of antioxidative action of the Rhodiola rosea extract

Investigation object: Rhodiola rosea extract, blood, liver, stomach of white nonlinear male rats. Investigation purpose: to establish the mechanism of antioxidative action of the Rhodiola rosea extract, its influence on the state of main antioxidants of rats blood, liver and stomach under conditions of physiologic norm and free-radical pathology models. Investigation methods: biochemical investigations (contents of lipid peroxidation products, oxidative modificated protein, general antioxidative activity of plasma, content of НS-groups; glutathione reductase, glutathione-S-transferase, glutathione peroxidase, glucose-6 phosphate dehydrogenase, catalase, superoxide dismutase, ceruloplasminum activities), histological investigations of liver and stomach tissues of rats. Theoretical and practical results, novelty of the investigation: for the first time the effect of Rhodiola rosea extract on the state of oxidative and antioxidative system of rats blood and liver in vitro and in vivo was investigated. Itwas proved, that Rhodiola rosea extract has strong antioxidative properties due to suppression of free-radical oxidation processes and activation of antioxidative defense in rats' blood, liver and stomach. For the first time the biochemical mechanisms of antioxidative action of the Rhodiola rosea extract were substantiated under conditions of physiologic norm and pathology models - experimental toxic hepatitis, gastroduodenal ulcer: suppression of free-radical oxidation of biomolecules due to decrease of concentration of reactive oxygen species; increase of membrane resistance and activation of antioxidative defense in rats blood, liver and stomach. For the first time the effective doses of the Rhodiola rosea extract were substantiated. Degree of application: this findings are applied to educational process in departments of pharmacology and pharmacy of Bucovinian State Medical Academy. Area of application: medical biochemistry.