Slyvchuk Y. Oxidation-Reduction Processes in the Mitochondria of the Tissues of Heifers and Cows in the Connditions of Polyovulation Produced by Gonadotropins

Object of research - the morphofunctional status of uterus, ovaries, liver, cardiac muscle and adrenal glands of cows and heifers in various metabolic conditions. Purpose - to establish the level of energetic processes in the mitochondria isolated from the tissues of some visceral organs of puberal heifers and cows under conditions of different patterns of the polyovulation induction by gonadotropine preparations. Methods of research and the equipment - biochemical, polarographic, microscopic, morphometric, gynecologic, polarograph LP-7, spectrophotometer CF - 26, microscope MBI - 10, MBI-15. Novelty - the influence of the polyovulation induced by gonadotropic hormones on the oxidation and phosphorylation processes in the investigated tissues of cows and heifers has been proved for the first time in an in vivo research. It has been established that the level of ascorbic acid in some interior and reproductive organs varies depending on generative changes in the ovaries. The advantage of the use of thecomplex hormonal preparation "Polyfol" containing gonadotropins and vitamins A, D3, Е, in comparison with the pure folliculostimulating hormone (FSH), has been demonstrated, since it enables reducing the labor input by 3 times and as well as lowering the costs of the polyovulation induction in cows-donors. The influence of the supplementary introduction of mineral-vitamin additives to the diet of cows-donors on the level of polyovulation induction has also been investigated. Implementation - the developed patterns of the polyovulation induction can be used during the preparation of cows-donors for the transplantation of embryos. Branch of application - biochemistry, biotechnology of reproduction of farm animals.