Vityukova K. New analytical forms based on the ternary complexes of europium (III) ions with some antibiotics

Оbject of investigation: ternary complexes europium (III) ions with cephalosporine and tetracycline antibiotics. Aim of investigation: search of new analytical forms based on the ternary complexes of europium (III) ions with some antibiotics for development of new high-sensitive luminescent methods for determination of components of ligand environments.Methods of investigation and instruments: spectrophotometry and luminescence spectroscopy. Results: some new analytical forms of the multi-purpose use have been found among complexes of europium (III) ions with cephalosporine and tetracycline antibiotics: ternary complexes Eu - methacycline - Н2О2 (sodium laurylsulfate), Eu - oxytetracycline - Н2О2 (citrate ions), Eu - chlorotetracycline - trioctylphosphine oxide, suitable for high-sensitive luminescent determination of antibiotics as well as second ligands (hydrogen peroxide, laurylsulfate and citrate ions). It is revealed, that in luminescence spectra of ternary complexes Eu - tetracyclines - Н2О2 intensity of forbidden transitions 5D0 --> 7F0 lines unusually high that is connected with increase by distortion of coordination polyedr Еu (III) at the inclusion of Н2О2 in the inner sphere of a complex and low symmetry of the luminescence centers, not above С2v. The methods of luminescent determination of cephtriaxone, methacycline, oxytetracycline,? chlorotetracycline in biological liquids, oxytetracycline in milk, citrate ions in pharmaceutical preparations, hydrogen peroxide in water, sodium laurylsulfate in washing-up liquids, and also a methods of indirect determination of glucose in blood plasma have been developed on the basis of new analytical forms. Field of application: bioanalytical chemistry.