Salamanova N. Differentiation of acid properties and salt effects in direct and reversed microemulsions

Objects of investigation: hydroxyxanthenes, microemulsions of different types. The aim: elucidation of the main influence regularities of microemulsions on the substances properties dissolved in microemulsions by means of hydroxyxanthene dyes. The methods: spectrophotometry, spectrophluorimetry, potentiometry. Scientific novelty: The indices of apparent dissociation constants of fluorescein and its derivatives were determined in direct and reversed microemulsions. Ionic equilibria of the same dyes were studied in the ternary solvent mixture. The monoanion HR– of fluorescein with non–ionized phenolic group was revealed in solutions. The dissociation constants of fluoresceinisothiocyanate in enzyme solutions has been obtained. Application: the data about apparent dissociation constants of hydroxyxanthene dyes give a possibility to make their application in analytical praktish more rational.