Arnauta O. Data of blood cells' metabolism and their level of undamaged state in circumstances of blood preservation by the factors of artificial hibernation

Ph.D. thesis contain the results of investigation of changes of some important biochemical and hematological data of animal blood (bulls and rabbits) in circumstances of its preservation using the medium with high concentrations of NaHCO3 and СО2 and also in standard glucose-citrate medium - "Glugicire". Transfusions of preserved blood to the animals-recipients have also been performed. It was found that in circumstances of blood preservation by the factors of artificial hibernation the intensity of glycolysis, the main metabolic cycle of blood cells, decreases essentially, that appears in less level of glucose consumption and, accordingly, in less lactate production in comparison with reference samples of blood. Investigated blood samples are characterized by more constant gradient of electrolytes concentration between the blood plasma and its cells; the data of acid-base status has changed essentially that was caused by the features of investigated medium composition; activity of some transaminasesin plasma of preserved blood samples investigated was much less in comparison with reference samples. In circumstances of artificial hibernation not only safe keeping of blood cells but also stability of their morphological and functional status was found that leads to a greater therapeutic effect of transfusion such a blood to animals-recipients. All the above mentioned demonstrate the essential impact of investigated factors of donor blood preservation on its metabolism and as a result - prolongation of animal blood storage term for 6-7 days, in comparison with preservation using standard glucose-citrate medium - "Glugicire". The results obtained can be recommended for introduction new bicarbonate and carbonic preserving medium into veterinary practice especially concerning the field of small animals cure. The data from the thesis can also be used for development of new and modification of existing blood preserving mediums by introducing carbonic components into their content.