Marchenko N. Affinity-chromatography analysis of chaperonins in normal conditions and under influence of cadmium ions.

Українська версія

Thesis for the degree of Candidate of Sciences (CSc)

State registration number

0407U002183

Applicant for

Specialization

  • 03.00.04 - Біохімія

21-05-2007

Specialized Academic Board

Д 240.26.01

Essay

Chaperonins. Elaboration of the method of affinity chromatography (ACh) of chaperonins of cells of different organisms. Main methods: spectroscopy, electrophoresis, chromatography. The method of analysis of chaperonin content in different organisms by the ACh was proposed; conditions of interaction of GroEL with different denatured proteins was investigated; the method of ACh on the basis of denatured pepsin was applied for isolation and purification of GroEL. Field of application - research institutions of biological and medical profile.

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