Kocheshkova N. Identification and properties of ion-transporting ATP-hydrolases of man's spermatozoa in the conditions of oligozoospermia.

The object – functioning of ATPases systems in man’s spermatozoa. The aim is identification of ATPfses, which have extra- and intracellular localizations of their active centres, their properties, peculiarities of functioning in normal and oligozoospermia conditions in man’s spermatozoa. Methods – enzymatic, specrtophotometrical, laser-correlation spectroscopy, light and electron microscopy, kinetics, statistical. Novelty – for the first time complex investigations of functioning of Са2+-depended and Са2+-independent of "intro"-АТРases in normal and oligozoospermia conditions have been conducted. There was defined the optimal regime of reaction of Mg2+-dependent enzymatic hydrolysis of АТР in spermatozoa. It was identified several "extra"- and "intro"-АТР-hydrolysing systems in male gamete’s membranes. The kalixarenes C­97 and C­107 inhibited the enzymatic activity of Na+,K+­ATPase more effectively than ouabain. There was suggested new biochemical diagnostic test for oligozoospermia. It is based on the increase of "common" ATPase activity in health men’s spermatozoa (comparing to the control without detergents) and the absence of its significant increase in the case of oligozoospermia.