Prylutska S. Biochemical effects of fullerenes C60 and C60-composites in the cells of various types

Dissertation is devoted to а comparative study on biochemical effects of fullerenes С60 and С60-composites in normal (rat thymocytes) and tumor (Erlich ascite carcinoma (ЕАС) and leucosis L1210) cells after UV-visible irradiation. Fullerenes С60 (10-5 -10-6 М) appear to penetrate into bilayer lipid membrane and to increase locally its conductivity. It is shown that fullerenes С60, С60-composites-1 and С60-composites-2 at С60 final concentration in probes 10-5 М have no cytotoxic and haemolytic effects - do not influence the viability of thymocytes, L1210 and EАC cells, accumulation of lipid peroxidation products or parameters of erythrocytes haemolysis. The intensification of reactive oxygen species (ROS) production in cell suspensions irradiated (320-580 nm) in the presence of fullerenes C60 is proved by EPR method. ROS production was increased in a sequence C60 < С60-composites-1<С60-composites-2. The damaging effects of photoexcited C60 appeared to be selective and to be manifested in transformed cells, but not in thymocytes. The biochemical mechanisms of photoexcited fullerenes C60 action are ROS production, early intensification of lipid peroxidation in cell membranes, inhibition of mitochondrial electron-transporting chain, DNA fragmentation and decrease of the number of viable L1210 and EAC cells.