Khmel T. The influence of N-stearoylethanolamine on the lipid composition of malignant and conditionally normal cells

Object: the biochemical parameters and processes in tissues of tumor and of tumor-bearing mice. Aim: to study the influence of NSE on transformed cell development in vivo and in vitro and to investigate the lipid composition of malignant cells and conditionally normal cells under carcinogenesis for elucidation of possible mechanisms of realization of the biological effects of NSE. Methods: methods of experimental oncology, of the cell cultivation, of spectrophotometry, methods of lipid analysis (thin-layer and liquid-gas chromatography ), electrophoresis.it was shown For the first time that NSE arrests the growth and metastasis of Lewis carcinoma in mice at the different stages of tumor development. We demonstrated that NSE disturbs the antioxidant protection of cells of the Lewis carcinoma and at the same time it inhibits processes of peroxidation in the adjacent conditionally normal lung tissue. We also have found that under tumor development N-stearoylethanolamine modulated the lipid composition ofthe adjacent lung tissue. NSE normalized the phospholipid content as well as the level of free cholesterol. Simultaneously NSE changed the lipid organization of malignant cells. The analysis of experimental data has shown that NSE was possessed the protective properties: it disturbed the metabolism of malignant cells and improved the state of tumor-bearing animals. N-stearoylethanolamine was shown to decrease the toxic effect of antitumor cisplatin treatment.