Boreiko O. Extraction and characterization of the proteins from the skeletal elements of sponge Suberites Domuncula.

Українська версія

Thesis for the degree of Candidate of Sciences (CSc)

State registration number

0408U002575

Applicant for

Specialization

  • 03.00.04 - Біохімія

20-05-2008

Specialized Academic Board

Д 26.001.24

Taras Shevchenko National University of Kyiv

Essay

The dissertation is devoted to investigation of biochemical properties of proteins from siliceous skeletal elements of sponge Suberites domuncula, and also to investigation of influence of these proteins as a part of biocomposit material on proliferation and mineralization in osteogenic cell culture SaOS-2.It was determined, that the proteinous part of the skeletal elements of the sponge Suberites domuncula predominantly consists of the proteins with molecular weght 23 and 24 kDa. The protein with molecular weight 24 kDa (silicatein ?) was identificated by electrospray ionization mass spectrometry method.

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