Haddad A. Age peculiarities of protein and lipid free radical oxidation in the brain of rats with hypothyroidism and effect of exercises to them

The object of study: free radical processes and the first line antioxidant defense enzymes in brain during experimental hypothyroidism of differently aged rats in the norm and during exercises. The aim of study: assay of age peculiarities of oxidative stress formation in the rat's brain with experimental hypothyroidism and during exercise as well. Methods: spectrophotometry, fluorimetry, differential centrifugation, immune-enzyme assay, statistical analysis. Theoretical and practical results, newness: the results obtained show that hypothyroidism is accompanied by oxidative stress formation in the rat's brain. It is manifested by an increase in concentration of lipid peroxidation products and protein carbonyls in mitochondrial and microsomal subcellular fractions of the brain. An increase in free radical generation in mitochondria during hypothyroidism plays a pivotal role in oxidative stress formation. The value of oxidative stress manifestation is greater in the brain of 12-months-old rats with hypothyroidism than in 1,5- months-old ones. Hypothyroidism in adult and pubertal rats promotes to a decrease in prooxidant effects exercise on the brain. The sphere of application: research of age-related pathology mechanism, lecture course of biochemistry, patholgical physiology.