Gilchuk I. The effects caused by injection of human apolipoprotein A-1 transgene into mammalian cells

The object is the expression of human APOA1 transgene in vitro and in vivo; the aim is investigation of APOA1 expression under the control of different strong promoters in CHO-K1 cells; the study of expression, the clearance time and the distribution of human APOA1 DNA in animal organism; the methods of microbiology, molecular biology, immunochemistry and biochemistry were used; the novelty lies in the construction the new recombinant plasmids, containing whole genomic variant of human APOA1 gene under the control of hCMV-ІA and CAG promoters; in examination of the clearance time and the distribution of human APOA1 gene in animal organism in the case of transgene delivery into the liver parenchyma in the complex with PEI; results include the expression of human gene under the control of different strong promoters in vitro and determine the clearance time, the localization and the expression level of APOA1 in vivo; the field is molecular genetics.