Daniel A. Contribution of maintenance conditions to genome stability in Rauwolfia serpentina Benth. cell strains

Українська версія

Thesis for the degree of Candidate of Sciences (CSc)

State registration number

0408U003149

Applicant for

Specialization

  • 03.00.22 - Молекулярна генетика

24-06-2008

Specialized Academic Board

Д 26.237.01

Institute of Molecular Biology and Genetics of NAS of Ukraine

Essay

The object is genetic variability in tissue culture of tropical plant R.serpentina; the aim is study of genetic stability of R.serpentina strains; the methods are tissue culture, molecular-genetic; the novelty lies in establishment of the fact that long-term culturing of R.serpentina strains in the stable conditions does not induce the genome rearrangements, while the changes of culture conditions might cause genome alterations; switch from the surface maintenance to submerged culture appears to influence more noticeably on the genome of cultured tissues as compared with the changes in solid medium composition.

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