Petruh I. Physiological prion and proteins of prion-replication organs depending on the age and feeding factors

Object: correlation of physiological prion isoforms, dynamics of brain lipids and prion-replicating organs of different breed cattle and mice, characteristic features of prion-protein zone lipids in the medulla of different breed cattle. Goal: to reveal the physiological prion and establish lipid characteristic features in prion-replicating organs of the animals depending on age, breed and feeding ration. Methods: spectrophotometric, electrophoretic, immunoelectrophoretic, western-blot, zootechnical, statistical analysis. Novelty: the correlation of separate physiological prion isoforms in prion-replicating organs (medulla, spleen, underjaw lymph node, mucous membrane of the bowels) and leucoconcentrate of cattle was investigated for the first time. The highest level of physiological prion was revealed in the medulla, lower - in spleen and underjaw lymph nodes, the lowest - in the bowels mucous membrane. The new data concerning PrPc isoforms in the in prion-replicating organs of the Ukrainian black-and-white dairy breed cows were obtained. Three isoforms of prion-protein were established: 2-, mono-, and deglicosilated. The 2-glicosilated isoform dominates in the medulla, spleen, underjaw lymph node and deglicosilated - in the bowels. The immunoelectrophoretic method of prion-protein detection was improved.