Medvedjev V. The effect of synthetic macroporous hydrogel implantation and olfactory bulb cells transplantation upon the spinal cord regeneration processes after its expiremental traumatic injury

The dissertation is dedicated to a problem of spinal cord restoration after experimental traumatic injury. The restorative effect of synthetic macroporous hydrogel implantation and allogenic olfactory bulb' cells transplantation (OBCT) after left-side hemisection (LH) of male rats' (age - 5,5 m; weight - 250-300 g) spinal cord were studied. A hydrogel was implanted into the LH zone immediately. OBCT was made in 4 and 8 weeks after hydrogel implantation and in 7 and 13 weeks after LH only into the spinal cord tissue below the LH site. At the 16th week hydrogel implantation leads to significant hind limb function improvement in 2-2,44 grades of BBB scale. OBCT in 4 weeks after hydrogel implantation and in 7 weeks after LH leads to the appearance of the significance peak of speed of ipsilateral hind limb function increase at the 4th-6th weeks after transplantation. Hydrogel implantation was accompanied by distinctive trisegmental dynamics of electrical activity indices: growth phase (1-7 weeks); stabilization (7-23 weeks, it is absent in the case of isolated LH); decompansation, peak and regression (24-31 weeks). OBCT leads to explicit reduction of excessive electric activity in efferent part of locomotor system.