Gorishniy M. Ecological significance of green sulphur sacteria in the utilization of sulphur hydrogen

The thesis presents the pioneering research into anthropogenic influence on the situation in Lake Yavorivs'ke. Relying on the known boundary values of the acceptable concentration of certain compounds and having compared Lake Yavorivs'ke with the natural control reservoir, it has been established that according to a number of characteristics the water of Lake Yavorivs'ke is hardly suitable for the existence of animated organisms. The dominant microorganisms identified in the water and mule of Lake Yavorivs'ke are typical saprophytes and oligonitrofiles. A number of sulphate reconstructing bacteria have been found in the mule. The number of phototrophic sulphur bacteria in the water and mule of the lake considerably exceeds the number of these organisms in the control reservoir. From the water of Lake Yavorivs'ke there has been extracted a pure culture of green photosynthesizing sulphur bacteria which perform detoxication of sulphur hydrogen. The extracted bacteria have been identified as C. limicolа Ya-2002. It has been discovered that the cells of C. limicolа Ya-2002 contain the following pigments: bacteriochlorophiles c and d along with carotenoids of chlorinebachene and isorenieratene. It has been revealed that the maximum growth of the culture took place under the exposure to red light with the length of wave amounting to 700?800nm and intensity of light amounting to 40lx. The intensity of light causes variations in the photosynthesizing apparatus of the cells, in the structure of chlorosomes of the culture in particular. It has been found out that the intensity of detoxication of sulphur hydrogen by means of green sulphur bacteria increased with the simultaneous introduction of piruvate and acetate into the environment on condition of the presence of hydrocarbonate of sodium. While cultivating C. limicola Ya-2002 in the environment with carbonic acid, piruvate and acetate and lighting of the culture with the length of radial wave of 700 - 800nm and intensity of 40lx there has been observed the maximumutilization of sulphur hydrogen amounting to 0,028 мМ per hour/100мg cells. Additional introduction of Н2S into the environment restored the growth of the culture. The principal inductor of the utilization of sulphur hydrogen by the cells of C. limicola Ya-2002 is light. It has been revealed that the primary carbohydrate product for C. limicola Ya-2002 in the process of assimilation of carbonic acid is glucose, which in the light rapidly turns to glycogen, the latter being extracted from the cells of C. limicola Ya-2002 and identified by means of infrared spectrometry. For the first time there have been considered the conditions of accumulation of glycogen by the cells and revealed the key role of lighting of the culture and participation of СО2 and sulphur hydrogen in this process