Becerril A. Molecular heterogeneity of the enzymes of carbohydrates metabolism in bone marrow cells and erythrocytes of rats submitted to the joint action of chronic low-dose ionising radiation and vitamin D3 dietary supplementation

The object of thе research are bone marrow cell populations and erythrocytes of rats. The aim was the detection of changes induced by low-rate radiation on the activity and isoforms distribuition of carbohydrates metabolism enzymes and the possibility of their correction by dietary supplementation with the vitamin-D3 containing solution "Videchol". Erythrocytes were lysed in liquid nitrogen. The bone marrow was fractioned in a gradient of ficoll-urographine mixtures. The bone marrow smears were stained according to Pappenheim and further studied under light microscope. The enzymatic activities and metabolites content were determined in the spectrophotometer SF-14. Lactate dehydrogenase's kinetics - in the spectrophotometer Spekol-11. The isoforms were separated by native disc-electrophoresis and their activity was revealed in visualization ractions. The densitograms were read in the micrphotometer MF-2. The statistical analysis was done using Studient's t-coefficient. The most significant changes ofactivity and isoforms distribution of carbohydrates metabolism enzymes upon a daily 1cGy dose occur at an absorbed dose of 20 cGy. Vitamin D3 led to normalization of enzyme's isoform activities. The isoforms redistribuition coefficient (lеі) was first time implemented in enzymatic systems. The results of the research could be used in biochemistry, radiobiology and medicine.