Silonov S. Participation of specific a-tocopherol binding proteins in the leucotriene synthesis regulation.

The objects of the investigation were blood and liver of rats. The aim of the investigation is the influence of tocopherol on both the oxidative metabolism of arachidonic acid on the leucotriens' biosynthesis in the rat organism at experiments in vivo and in vitro. Methods and eqvipment were used: spectrofotometric, chromatography (HPLC- Knauer, GLC-Carlo Erbe, thin-lauer chromatography, gel-filtration-liquid chromatograph GH1304, ELISA-Bio-Rad Microplate reader model 450 , liophilization -Inei 220. The new data about a-tocopherol mechanism action on the arachidonic acid metabolism associated leucotriene metabolism have been obtained. Condition of the different vit. E provision influenced on the lipid metabolism as consist of the tocopherol, cholesterol, phospholipids in rat liver have been explored. Intension 5-lipoxygenase way arachidonic acid metabolism at the arachidonic acid, cysteinil-leucotriens concentration in rat liver and blood have been explored. a-Tocopherol and tocopherylquinone complexwith a-tocopherol binding proteins (TBP) of the rat liver cytosol have been received. TBP in the regulation process by tocopherol leucotriene composition have been investigated. The influence both TBP complex with the a-tocopherol and TBP complex with the tocopherylquinone on the leucotriene enzyme activity in vitro experiment has been investigated . For the first time it was proved the possibility of the influence of tocopherol and tocopherylqinone in vitro on the investigated enzyme systems of regulation of arachidonic acid pathway. It was established the important functional role of tocopherol-binding proteins for the realization of the influence of tocopherol and tocopherylqinone on the enzymes which regulate the arahidonic acid metabolism.