Kosiakova G. The influence of N-stearoylethanolamine on the nitric oxide synthesis under the normal and different pathological conditions

Object - the biological action of N-stearoylethanolamine (NSE); aim – to investigate the influence of NSE on the nitric oxide (NO) synthesis by NO-synthase pathway under the different pathological conditions which is accompanied by disbalance of NO synthesis; methods – spectrophotometrical assay, method of lipids assay (thin layer chromatography and gasliquid chromatography), tonsimetric assay, kinetic and statistic analysis; the new approach – it was shown for the first time that NSE is able to regulate content of NO in organism under the different pathological conditions, induced by ischemia-reperfusion tissue damage, irradiation effects, diabetes mellitus. It was found that in base of NSE effect lays its ability to regulate NO-synthase activity. On the pathological experimental models and human lung hypertension, both of which have NO overproduction, NSE inhibits inducible NO-synthase izoform (iNOS) activity, both in vivo and in vitro studies. It was shown the ability of NSE to correct damage of phospholipids composition of cell membranes induced by disease development. The decrease of phospholipids lyzophorms content may provoke iNOS activity inhibition and this biochemical mechanism can explain NSE effect on enzyme activity; the effect of iNOS inhibition under the NSE action may be used for establish a new medicine on the NSE base; field of application – research institute of biological and medical profile