Kalashnik O. The effect of fibrin degradation products on PC-12 cells

Object – live function of PC-12 cell culturing in the presence of fibrin and its degradation products; receptors mediating the effect of high molecular weight fibrin fragments on PC-12 cells. Aim – studying of the mutual effect of a fibrin clot and PC-12 cells. Methods: microscopy, cytochemistry, SDS-electrophoresis, enzyme-electrophoresis, immunobloting, sorption and cell immunoenzyme assay, chromogenic assay, MTT assay, flow cytometry, kinetic analysis. It was shown for the first time that interaction between PC-12 cells and fibrin depends on volume and density of added clot. It was shown for the first time that low (10–30 kDa) and high (>30 kDa) molecular weight fibrin degradation products influence PC-12 cells in a different manner. Low molecular weight fragments stimulate cell proliferation, survival, processes outgrowth and nicotinic acetylcholine receptor expression. Soluble high molecular weight fragments (>30 kDa), including purified D, DD, E fragments, improve PC-12 survival and adhesion toplastic but slow cell division, while the immobilized ones, on the contrary, favor cell proliferation. The D, DD and Е fibrn(ogen) fragments influence on PC-12 cells is mediated by ?V?3 integrins. It was determined PC-12 basic production of tissue plasminogen activator. The established mode of interaction between fibrin fragments and PC-12 cells may be used for correction of fibrin glues applications in neurosurgery.