Maksymchuk O. Changes of a mouse liver Cytochrome P450 2E1 gene expression upon the exogenous' stresses

Object - molecular mechanisms of cell response upon the exogenous' stresses; Objective - to investigate expression of Cytochrome P450 2E1 gene in the mice liver at the separate and joint action of exogenous stress factors - ethanol and gamma-radiation; methods - methods of cloning of Cyp2e1 of mouse in the system E.coli, synthesis of polyclonal specific antibodies to Cytochrome P450 2E1, biochemical methods of determining the amount of microsomal Cytochrome P450, methods of analysis of protein and RNA, immunological analyses of protein, methods of mathematical statistics; novelty - influence of the long-term separate and joint action of low-intensive gamma- ray's and ethanol is first investigational on the level of expression of Cytochrome P450 2E1 gene in the mice liver. Тhe decrease in CYP2E1 protein levels and mRNA levels of expression upon the long-term exposition of low doses of gamma-irradiation was observed. The acute highly-intensive gamma- ray's exposure of small doses increased but the largedoses exposure decreased CYP2E1 protein contents while levels of Cyp2e1 mRNA expression were down regulation at any doses applied. The chronic ethanol treatment induced the CYP2E1 protein expression but did not affect the mRNA profile. The initial increase in the enzyme content observed after the two stress' factors have been applied simultaneously might be due to the stabilizing action of ethanol. It have been evaluated that the CYP2E1 protein content as well as the level of mRNA gradually decreased upon the gamma-irradiation extension.