Zasadnyuk I. The effect of autologous mesenchymal stem cells on the articular cartilage regeneration (experimental examination)

The thesis is devoted to the study of effect of autologous mesenchymal cells of bone marrow with various degree of chondrogenic differentiation on the course of reparative chondrogenesis of the articular cartilage with its traumatic injury. The optimal density of inoculation and terms of previous culture of autologous mesenchymal cells of bone marrow for their cultivation and chondrogenic differentiation have been determined. It has been proven on the basis of performed biochemical examinations that the intra-articular infusion of autologous mesenchymal cells of bone marrow at the earlier stages of pathologic process stabilizes the metabolic processes in cartilaginous tissue, and farther on normalizes them reaching the physiologic norms that are characteristic for intact animals. The practical value of the examination results consists in argumentation of high efficiency of the articular cartilage treatment with the aid of autologous mesenchymal cells of bone marrow in experimental animals and the experimental substantiation of the use of autologous mesenchymal stem cells of bone marrow in the treatment of orthopedic and traumatologic patients