Grabovetskaya E. Age-related peculiarities in the activity of aldehyde scavenger enzymes in rat's heart during immobilization stress

The object of study - process of endogenous aldehydes catabolism in heart of intact and immobilized rats of various ages. Purpose - to research activity of endogenous aldehydes scavenger enzymes in myocardium subcellular fractions of rats of various ages and to examine peculiarities of enzymatic activity during immobilization stress. Methods: spectrophotometry, fluorimetry, differential centrifugation, immune-enzyme assay, statistical analysis. Theoretical and practical results, novelty: the obtained results show certain changes in the activity of aldehyde dehydrogenase, aldehyde reductase and glutathione transferase in the mitochondrial and postmitochondrial fractions of the heart muscle during ontogenesis. Under immobilization stress, the modulation of the activity of endogenous aldehydes scavenger enzymes in subcellular fractions of the hearts of pubertal rats appears to be stronger than those of adult and old ones. At the same time, 1,5- and 2-month old rats have an increased level of TBA-reactive substances and Schiff bases in mitochondrial and postmitochondrial fractions of myocardium. Older pubertal rats show higher levels. These alterations indicate inhibition of endogenous aldehydes scavenge in pubertal rats' heart during stress. Field of application: research of age-related heart pathology mechanisms, lecture courses in biochemistry, physiopathology.