Kravchenko G. The influence of phytogenic antioxidants on metabolism of the nitrogen-containing compounds under the neurogenic stress in rats

Research object - biochemical mechanisms of neurogenic stress development in rats. Research aim - to determine plant antioxidants influence on nitrogencontaining compounds metabolism under neurogenic stress in rats. Research methods: Spectrophotometric determination of main nitrogen metabolism products and activity of nitrogencontaining compounds metabolism enzymes. Immune-enzyme analysis of corticosteroids. Theoretical and practical results, innovations. It was shown, that as neurogenic stress caused by immobilisation and the introduction of epinephrine high doses, lead to arginine level decrease both in the blood and tissues of the animals that is correlated with the increase of arginase activity. The activation of urea synthesis is also proved by the increase of the urea contents in the blood of stressed animals. In addition the lack of arginine caused the creatine deficiency, which is necessary for phosphocreatine synthesis. In this conditions the phosphocreatine degradation is activated that indicated by creatinine level increase in animal blood and liver. The used models were also accompanied by cytolysis activation because of free-radical damage of the membranes. This caused the observable hyperenzynemia. The uric acid increase was common for the used models. In general the introduction of Grape seed extracts stabilized cell membranes that were manifested by cytolysis activity decrease and catabolism development. Obviously, the differences in extracts actions are connected with different points of their components influence on the cell.