Galat M. Analogous alkyl-bonded stationary phases in micellar and reversed-phase liquid chromatography

Object of investigation: the process of saqmple resolution by using micellar liquid chromatography (MLC) and reversed-phase high performence liquid chromatography (RP HPLC). Aim of investigation: creating the approach to the classification of octedecylsilica columns which aloow to choose column with equal selectiwity whin MLC is used; to developed and validate the method for the flavonoids determination in medicin plants and drugs with medicin plant extract by using MLC. Methods of investigation: potentiometry for the critical micellar concentration determination and degree of counterion relationships for sodium dodecyl sulfate (SDS); micellar liquid chromatography; reversed-phase high performence liquid chromatography. Theoretical and applied results: the approach and selectivity parametrs based on different retention mechanisms allows chosing equal according to selectivity columns when MLC is used for the sample separation. The availability of MLC using for the simultaneous separation of hydrophobic and hydrophylic flavonoids. Science novelty: first C18 column classification based on parameters of sample separation with different retention mechanisms was proposed when MLC is used. It was founded that in MLC mode individual C18 column properties were less then the same columns used in RP HPLC mode. Firstly founded that 1-buturic and 1-pentanoic acids influence the same modification effects on SDS micellar properties as equal aliphatic alcohols. Degree and field of the practical application: method of luteolin-7-O-glucoside and apigenin-7-O-glucoside assay in the matricaria flowers was adoption and used in the Laboratory of Pharmacopoeial Analysis in "Ukrainian Scientific Pharmacopoeial Centre of Drug Quality" (Kharkov).