Chorna M. The influence of cadmium and cobalt salts on origin of oxidative stress for white rats and correction of violations of metal complex and enterosorbent "Fibrabet"

The object - toxic poisoning in rats with cadmium chloride, cobalt chloride and their combination. The aim is to find out the influence of cadmium and cobalt salts intoxication on the formation of active forms of oxygen and the course of free radical processes in the blood and liver of rats, to explore the possibility of correction of these violations by the help of metal complexes and enterosorbent "Fibrabet". Methods - biochemical (spectrophotometric), chemical (synthesis of metal complexes of amino acids), UV spectroscopy (determination of zinc tyrosinate spectrum absorption), statistics (the method of variation statistics using the Student t-criterion). Novelty - the poisoning of animals due to a combination of cadmium chloride and cobalt chloride intensifies the formation of active forms of oxygen (superoxide anion radical, hydroxyl radical, hydrogen peroxide ) and the activation of free radical oxidation of lipids and proteins was found in this work for the first time, based on complex investigation. Corrective influence of prednisolone, copper tyrosinate, zinc tyrosinate, zinc histydynate and magnesium glutamate with vitamin B6 on the formation of radicals was studied and the greatest efficiency under input zinc tyrosinate was found. For the first time shown that introduction of this metal complex decreases indexes of free radical oxidation of lipids and proteins in both plasma and liver of affected animals. More effective influence of zinc tyrosinate and enterosorbent "Fibrabet" combined action have been found, applied for correction of biochemical disorders, caused by simultaneous introduction of cadmium and cobalt salts.