Chornyy S. Influence of thiamine antagonists on apoptotic signaling pathways activated in nerve cells

Object: signaling processes in nerve cells due to thiamine deficiency. Aim: To study the influence of thiamine antagonists on the viability of subcultures of nerve cells of different types of organisms and determine the processes that lead to their death. Methods: biochemical, molecular-biological, immunochemical. It was established that in nerve cells thiamine antagonists are capable to induce mitochondria-mediated non-receptor-reliable caspase-dependent apoptosis in the activation of which the key role is played by p53 protein. Thiamine antagonists promote the expression of genes of proteins involved in metabolism and functioning of thiamine (thiamine transporter THTR-1, pyruvate dehydrogenase, alpha-ketoglutarate dehydrogenase and transketolase). There was established a direct correlation between expression of genes TDP-dependent enzymes, THTR-1 and active p53 protein in neuronally differentiated SH-SY5Y cells. Obtained results indicate that p53 protein influences on the level of gene expression of the enzymes mentioned above. Field of application-scientific institution of medical and biological type.