Demkovich A. Molecular genetic peculiarities of Scotch pine plants with high productivity and pathogen resistance and they progenies

Українська версія

Thesis for the degree of Candidate of Sciences (CSc)

State registration number

0410U004816

Applicant for

Specialization

  • 03.00.22 - Молекулярна генетика

23-11-2010

Specialized Academic Board

Д 26.254.01

Institute of Food Biotechnology and Genomics of the National Academy of Sciences of Ukraine

Essay

A molecular-genetic diversity of plus- and resistant against Annosus root rot and air pollution Scotch pine trees have been investigated. Isozymes and nuclear microsatellites DNA used as genetic markers. For pines which persisted in the clusters of a extreme pathogen lesion allelic and genotypic diversity lover, than in natural populations. The persisted plants are in genotypic equilibrium according Hardy-Weinberg law whereas their embryos samples and natural populations in genotype disequilibrium due to excess of homozygous genotypes and low outcrossing rate in clusters of a extreme pathogen lesion. Mineral Nitrogen emissions case low impact by diversity Nitrogen metabolism allozyme loci of in embryos of Scotch pine and their mating. The values of molecular-genetics polymorphism plus-trees not different from native populations of Scotch pine, but our allelic and genotypic structure hawed differences by individual isozyme loci (Got-1, Mdh-4) and alleles Got-30.60, Dia-41.00, Acp0.94. The plus-trees have high outcrossing level, their progenies are in genotypic disequilibrium according Hardy-Weinberg law due to excess of heterozygotes. Variability of nuclear microsatellite loci in trial cultures are similar to parental trees due too pollen gene flow. A plus- and resistant against Annosus root rot Scotch pine trees have differences by allozyme loci

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