Kulitska M. The peculiarities of prooxidant-antioxidant balance in rats under the effect of heavy metals and sodium nitrite and correction of violations

The object - toxic poisoning in rats with cadmium chloride, lead chloride and sodium nitrite. The aim is to find out the features of lipid peroxidation and antioxidant system in rats with combined affection of cadmium chloride, lead chloride and sodium nitrite, to explore the possibility of correction of these violations by the help of metal complex copper histidinate, enterosorbent "Enterosgel" and isolated hepatocytes. Methods - biochemical (spectrophotometric), chemical (synthesis of metal complexes copper histidinate from histidine), statistics (the method of variation statistics using the Student t-criterion). Novelty - the poisoning of animals due to a combination of cadmium chloride, lead chloride and sodium nitrite intensifies the processes of free radical oxidation of lipids, decreases activity of antioxidant system and endogenous intoxication was found in this work for the first time, based on complex investigation. Developed new methods of correction of these violations, based on the usage of metal complex copper histidinate, enterosorbent "Enterosgel" and isolated hepatocytes. For the first time shown that introduction of "Enterosgel" decreases manifestations of toxic effects of heavy metals salts and sodium nitrite, decreases indices of free radical oxidation of lipids and normalizes the activity of antioxidant system in both plasma and liver of affected animals. For the first time was investigated influence of isolated hepatocytes on biochemical processes in affected animals, which leads to normalization of antioxidant enzymes function and decreasing of endogenous intoxication. More effective influence of "Enterosgel" and isolated hepatocytes combined action have been found, applied for correction of biochemical disorders, caused by simultaneous introduction of cadmium chloride, lead chloride and sodium nitrite.