Fylymonenko V. 3. The heme oxygenase activity regulation by heme in rat heart, vessels and lung under NO-synthases substrate and inhibitor administration

The object of study - regulation of heme oxygenase activity. Purpose - to ascertain general pathways of heme oxygenase activity regulation by heme under NO-synthases substrate and inhibitor administration. Methods: differential centrifugation, differential spectrophotometry, photocolorimetry, statistical analysis. Theoretical and practical results, novelty: the obtained results show the total heme accumulation and heme oxygenase activity increase in rat heart, vessels and lung under glycerol rhabdomyolysis. Pretreatment by L-arginine was established to cause the earlier heme oxygenase activity increase and to show antioxidative action in these organs that permit to regard pretreatment by L-arginine as one of ways of heart and lung injuries limitation under significant heme accumulation in blood. It was established the hemin chloride administration causes the heme accumulation and heme oxygenase activity increase in vessels but don't act on these parameters in rat heart and lung. Pretreatment by N -nitrо-L-arginine was shown to strengthen heme oxygenase activation in vessels caused by hemin injection. Field of application: research of mechanisms of heart, vessels and lung injuries under heme accumulation in blood and search of correction ways of cardiovascular system and lung metabolism in these conditions; lecture courses of biochemistry, physiopathology.