Gidulyanova K. Features of evince the effect of induced oxidative stress in erythrocytes of children and adolescents

The object of study. Process of lipid peroxidation, the activity of enzymes of the first line antioxidant protection, phospholipid structure of erythrocyte membranes of children and adolescents under conditions of induced oxidative stress. Methods: spectrophotometry in seen and near ultra-violet area of a spectrum, thin-layer chromatography, gas chromatography, statistical methods of the analysis. Theoretical and practical results, novelty: in erythrocyte membranes and hemolysates of children and adolescents at 1-hour induced lipid peroxidation there are the increase in the level of di- and triene conjugates and increase in superoxide dismutase and catalase activities in hemolysates in comparison with basal values. At 2-hour influence of Fenton solution in erythrocyte hemolysates of children it is kept increase in catalase activity on its reference level. Thus superoxide dismutase and catalase activities at adolescents come back to their reference values. After incubation erythrocyte with Fenton solution there are a decrease in the content of phosphatidylcholine and increase in the content of lisophosphatidylcholine concerning reference values in erythrocyte membranes, the most expressed at children. At induced the lipid peroxidation in fatty-acids structure of erythrocyte membranes of adults surveyed the expressed decrease occurs in a level of unsaturated fatty acids. After incubation erythrocyte with plasma of blood in erythrocyte membranes of adults surveyed the content of short-chain monoenic fatty acids raises and decreases their level of plasma of blood. All these changes reflect the increased sensitivity of erythrocyte of children to short, and adolescents to prolonged oxidative stress. Field of application: research of mechanisms of stability of cells to factors of oxidative stress, lecture courses in biochemistry and physiology.