Peretiatko I. The influence of the X-ray irradiation on the NO-dependent regulatory mechanisms regulation of the morpho-functional state of immunocompetent blood cells

The object–structural and functional state of leukocytes of kontrol and irradiated animals and irradiated in vitro immunocompetent blood cells, received from healthy donors. The subject–to find out an influence of chronic X-ray irradiation on morfological and functional state of leukocytes of peripheral blood of laboratory rats and find the ways to correction of processes of revealed violations. Methods of investigation: biochemical (spectrophotometric), molecular-biological (DNA-commet assay), cytological (immunocytochemic, morphology cell assay, method of blasttransformation of lymphocytes), statistics. It has been shown that the basis of the biochemical impairments which cause changes in structural and functional state of immunocompetent blood cells under chronic X-ray irradiation is formed due to significant increase in nitric oxide (NO) production induced by the change in oxidative - non-oxidative pathways of L-arginine metabolic intensity ratio at the expence of the increase of NO-synthase activity against the background of arginase inhibition. The activation of the apoptosis in the immunocompetent blood cells treated with irradiation is induced by nitric oxide hyperproduction. L-NAME as non-selective inhibitor of NOS proved to be the factor capable to prevent the programmed cell death of immune system cells by means of inhibition of toxic and proapoptic effects of nitric oxide under X-ray irradiation. It has been proposed to continue research on the basis of L-NAME for development of preparations which may prevent or correct the impairments that appear in the leukocytes of peripheral blood due to prolonged X-ray irradiation.